A. Research history
B. Vaccine make and procedures
C. Difference
D. Challenges
E. Important steps
F. Wrong vaccine and improper method
G. Impact of shaded and sunlight environment
H. Equipment required for vaccine application
Inoculation in Agar oud trees refers to the advanced process of introducing certain microorganisms, viruses, bacteria, or vaccines into the body of an Agar oud tree to enhance its immune response at specific sites.
Uninfected plants are inoculated with microbial culture. As a result, agar plants naturally produce Agar oud. Apart from this, Agar oud production is facilitated by introducing.
Sufficient amounts of virus/bacteria into the plant hole. Protozoa are single-celled organisms that are usually invisible to the naked eye and exist as cells or colonies of cells. Such as viruses, bacteria, algae, and fungi.
A. Research history
Anselm Payen 1859 attempted to produce Agar oud through the first chemical analysis of the agar plant. The Tunstall method of inoculation was developed in 1929 (20). Later, this practice spread to different countries. Invented by Dr. Robert A. of the CA Kit, University of Minnesota, USA. These methods have been tested in countries around the world, such as Bhutan, Myanmar, Thailand, Vietnam, and Laos.
Control Stick (Inoculation and Temperature) BANGLADESHIO S.A. Theory 2024NS The Theory is invented. With the improvement of the previous technology, the use of a Control stick as a new technology ensures the production of Agar oud.
In different countries of the world, vaccines are used to produce Agar oud on agar plants. Among the raw materials used in the production of vaccines or serum in different countries of the world today, it is notable-
Sodium bisulfate
Sodium Chloride
Ferric Chloride
Chitin
Formic Acid
Cellobiose
Salicylic Acid
Iron Powder
East Powder
Cetachu Etc
Vaccines or serums are prepared by combining two or more raw materials. Example-
France- Sodium bisulfate, Formic Acid
Vietnam- Sodium bisulfate, Yeast Powder, Iron Powder
Island- Sodium Chloride, Sodium Bisulfate, Ferrous Chloride
Bangladesh-
Bd Formula one vaccine (connect) Sodium Chloride, Yeast Powder, Cetachu
BD formula Nayla vaccine (connect)(Secret formula of Agar oud society Bengladeshio Culture)
B. Vaccine makers and procedures
BD Formula One vaccine (connect)
In the inoculation method for Agar oud trees, the mixture of yeast powder, salt, catechu can lead to the formation of various types of microorganisms. These microorganisms play an important role in the production of Agar oud, as the infection by these microbes initiates the process of agar formation within the wood.
Roles of Vaccine Ingredients:
1. Yeast Powder
This is a fungus-based organism that penetrates the plant tissues and causes infection.
As a response to the infection, the tree produces resin (agar) as a natural defense mechanism.
Yeast activates the plant’s biological response process.
2. Salt
Salt is not favorable for microbial growth, but in a precise amount, it helps degrade plant tissue.
When tissue breaks down, yeast spreads more easily, allowing the vaccine to work deeper.
3. Catachu (colour)
This is a chemical substance that induces artificial stress in plant cells through chemical stimulation.
Such stress triggers the resin production process.
It also contributes to a dark black color, which helps in internal cell and tissue coloration, making resin production easier.
4. Guaranteeing Agar oud Production:
For assured Agar oud production, the use of the BANGLADESHIO S.A. Theory 2024NS formula is mandatory.
All these microorganisms work together to cause connected changes inside the oud, which are essential for the formation of Agar oud.
C. Differenc
The main differences between chemical vaccines and connect vaccines in Agar oud production are:
1. Ingredients:
Chemical Vaccine: This is usually prepared using various artificial chemical substances. This vaccine accelerates the aging process of the Agar oud tree and speeds up the production of Agar oud.
Connect Vaccine: This is made entirely from natural ingredients, which helps to speed up Agar oud production without causing any harmful effects on the tree.
2. Impact:
Chemical Vaccine: The use of chemicals can damage the tree’s natural growth and the quality of the oud. While it yields quick results, it can have negative long-term impacts on the environment.
Connect Vaccine: This works gradually but is safe for the tree, promoting better tree health. The oud quality improves, and there is a higher yield of clone Agar oud.
3. Environmental Impact:
Chemical Vaccine: The increase in chemical substances in the environment can harm the soil, water, and other biodiversity.
Connect Vaccine: It is eco-friendly and does not create any negative impact on the environment in the long term.
4. Purpose of Use:
Chemical Vaccine: Used for increasing production quickly and supplying products to the market faster.
Connect Vaccine: Used with a goal of sustainable and natural production, prioritizing the tree’s health and environmental protection.
Chemical vaccines provide quick results but can disrupt natural balance, while connected vaccines are more sustainable and help in eco-friendly Agar oud production.
D. Challenges
Despite the use of inoculation methods in Agar oud production, there are still some challenges in producing high-quality Agar oud globally. Some of the reasons include:
1. Lack of Proper Technology: Often, producers lack the appropriate technology or modern equipment, which hinders the assurance of product quality.
2. Environmental Factors: Agar oud production requires the right environment, such as temperature, humidity, and airflow. If these conditions are not suitable, the quality may decline.
3. Species Diversity: Different species of Agar oud produce varying qualities. Some species may naturally yield lower quality.
4. Infectious Diseases: The absence of proper infectious agents in the inoculation process can cause damage to the production, weakening the quality of Agar oud.
5. Lack of Experience: Many producers work without adequate training or experience, leading to lower production quality.
6. Market Demand: If there is little awareness of quality in the market, producers may focus on low-cost production, leading to a decline in product quality.
Due to these factors, producing high-quality Agar oud remains a challenge, even when using inoculation methods.
E. Important Steps
Important Steps to Take Before Using the Inoculation Method on Agar oud Trees:
Steps:
1. Soil Testing: It is important to determine the correct nutrient levels and soil pH for Agar oud trees. Choosing the right soil is critical for successful inoculation.
2. Selecting the Right Species: To produce high-quality Agar oud, it is essential to choose the appropriate species of tree.
3. Evaluating Environmental Factors: Environmental factors such as temperature, humidity, and airflow should be assessed, as they play a significant role in inoculation and production.
4. Proper Inoculation Technology: The inoculation process must be applied correctly to ensure the infection process occurs smoothly and the trees grow healthily.
5. Training and Education: Producers should be trained and made aware of modern technology for improving production.
Advantages and Disadvantages of Using the Inoculation Method in Agar oud Production:
Advantages:
1. High-Quality Production: Inoculation methods can lead to specific and high-quality Agar oud production, depending on the quality and species of the inoculant.
2. Consistent Yield: Agar oud produced through inoculation tends to be more consistent and reliable, helping to meet market demand.
3. Increased Production Rate: The use of inoculation methods boosts the production rate of Agar oud, as it can grow rapidly.
4. Controlled Environment: Inoculation allows the production environment to be controlled, reducing the risk of diseases and pests.
5. Development of New Species: Inoculation methods make it possible to create new and improved species of Agar oud.
Disadvantages:
1. Expensive: The equipment and inoculants required for inoculation methods can be costly, increasing production expenses.
2. Requires Technical Expertise: Inoculation needs skilled experts to be executed correctly, which may not always be easily available.
3. Risk of Tree Rot: If the inoculation process is done incorrectly or unhealthy inoculants are used, the tree may rot or decay.
4. Disruption of Natural Process: Inoculation methods may interfere with natural processes, potentially harming the environment.
5. Requires Regular Monitoring: To ensure the effectiveness of inoculation, regular monitoring and maintenance are necessary.
In summary, while inoculation methods are an effective and profitable way to produce Agar oud, they come with some disadvantages. With proper management and the application of technology, these challenges can be addressed.
F. Wrong vaccine and improper method
Applying vaccines in Agar oud is a complex and scientific process. However, improper vaccine application or incorrect methods can hinder the production of agar. Below is a detailed explanation of some common mistakes made during the production of Agar oud through vaccine creation or application:
1. Using the Wrong Vaccine: It is essential to select the correct microbes or fungi to induce infection in Agar oud trees. Using the wrong fungi or microbes can result in not producing the desired fragrance or agar oil, or it may produce low-quality oil. In some cases, the tree may even be completely destroyed.
2. Incorrect Vaccine Dosage: If the vaccine dose or quantity is not correct, problems can arise. Excessive application of the vaccine may cause the tree’s cells to deteriorate too quickly, disrupting the production of agar oil. On the other hand, if too little vaccine is applied, the infection may not fully develop, leading to inadequate oil production.
3. Applying the Vaccine at the Wrong Time/Season: The correct timing for applying the vaccine is crucial for Agar oud production. If the vaccine is not applied at the proper stage of the tree’s growth, it will not create the desired effect. Typically, the appropriate time for vaccination is when the tree has matured to a certain stage.
4. Incorrect Injection Method: Following the proper method for vaccine application is essential to avoid damaging the internal structure of the tree. For example, if the injection is too deep or too shallow, it may not induce the desired infection or may damage the oud’s quality.
5. Wrong Injection Site: Vaccines should be applied to specific parts of the tree. If the injection is given in the wrong place, the infection may not spread correctly, and the desired quality of agar oil will not be produced. The best results are obtained when the infection occurs at specific parts of the tree trunk.
6. Errors in Vaccine Preparation: Mistakes can occur during the biological process of vaccine preparation. For instance, if the infection-causing fungi are not properly tested or controlled for quality, they may not work effectively or may harm the tree.
7. Ignoring Environmental and Climatic Factors: The effectiveness of the vaccine also depends on the climate and environment of the Agar oud tree. For example, in dry or cold environments, the infection may not occur properly. If the tree’s moisture or soil conditions are not favorable, the vaccine’s effect may diminish.
8. Neglecting Testing and Monitoring: Regular testing and monitoring are essential after the vaccine application. Often, if proper monitoring is not done after applying the vaccine, the infection may not develop correctly, or the tree may suffer damage. Without regular testing, issues with the tree may not be detected in time.
To avoid these mistakes, research and technology must be used in a coordinated manner, and vaccines must be applied according to proper procedures.
G. Impact of shaded and sunlit environments
Producing high-quality Agar oud or oil from Agar oud trees is a long-term process, primarily resulting from specific infections or injury-induced responses in the tree. Vaccine technology can accelerate and regulate this process, contributing to the production of superior-quality Agar oud resin. However, there are notable differences between light-shaded and sunlit areas that may impact the results when using vaccines. Trees in light-shaded areas tend to have higher nutrient content than those in sunlit areas. Below is a detailed explanation:
1. Effect of Vaccine Application on Agar oud Resin Production in Light Shaded Areas:
Tree Growth: Agar oud trees in light shaded areas generally grow at a slightly faster rate. The soil in shaded environments retains more moisture, and the cooler conditions reduce physical stress on the tree.
Resin Production: The internal environment of trees in light-shaded areas is relatively humid, which influences the resin formation process triggered by the vaccine application.
Outcome: Vaccine application in light shaded areas may result in slower resin production due to lower sunlight intensity. However, the resin produced is likely to be deeper and denser, offering higher quality Agar oud resin.
2. Effect of Vaccine Application on Agar oud Resin Production in Sunlit Areas:
Tree Growth: Agar oud trees in sunlit areas grow at a slower rate. The intense sunlight can dry out the soil and increase physical stress on the tree.
Resin Production: In sunlit environments, vaccine application prompts faster reactions in the tree due to the increased stress, leading to higher levels of infection.
Outcome: Resin production in sunlit areas is comparatively faster, but the depth and density of the resin may be lower. However, the oil produced is often of premium quality.
3. Comparative Results of Vaccine Application in Both Environments:
Production Speed: Resin production occurs faster in sunlit areas, whereas shaded areas require more time.
Quality: Agar oud resin produced in shaded areas tends to be denser and of higher quality, while resin from sunlit areas is relatively lighter.
Oil Quantity: Sunlit areas often yield more oil of high quality. In light shaded areas, the quantity might be comparable, but the resin’s density is higher.
The use of vaccines in light-shaded and sunlit areas may yield different outcomes. Light Shaded areas have a higher potential for producing high-quality Agar oud resin, while sunlit areas allow for faster production, though the quality may be slightly lower. By adopting proper strategies and regular monitoring, it is possible to achieve optimal results in both environments.
H. Equipment needed for vaccine administration
Generator, Drill Machine, Electric Wire/Instrument, Hard Pipe, Cutter, Vaccine & Water Level Pipe, Injection, Geometry Box, Spray Machine, Pesticide (Dustpan, Malathion), Vaccine, Bucket, Rope, Bamboo, Knife, Saw, Axe, Fertilizer, Inch tape, Control stick, Thermometer
